The first part shows that Du Bartas' friendship with James VI and I was key to his later popularity. Du Bartas' poetry symbolized a transnational Protestant literary culture in Huguenot France and Britain. Through James' intervention, Scottish literary tastes had a significant impact in England. Later chapters assess how Sidney, Spenser, Milton, and many other poets justified writing poetic fictions in reaction to Du Bartas' austere emphasis on scriptural truth. These chapters give equal attention to how Du Bartas' example offered a route into original verse composition for male and female poets across the literate population.

Du Bartas' Legacy in England and Scotland responds to recent developments in transnational and translation studies, the history of reading, women's writing, religious literature, and manuscript studies. It argues that Du Bartas' legacy deserves far greater prominence than it has previously received because it offers a richer, more democratic, and more accurate view of sixteenth- and seventeenth-century English, Scottish, and French literature and religious culture.