Description - Hybridization Techniques for Electron Microscopy by Gerard Morel
This text examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. It features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissues methods using ultrathin frozen sections, pre-embedded methods and post-embedded methods using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid and detection of messenger RNA in animals and plants.
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