The first type is most often encountered in practice. Two molecular materials may be made that have different average molecular masses, or a broad molecular mass material may be separated, often chromatographically. In such situations when the mixtures are indistinguishable and overlapping, moments of the molecular mass distribution of the pure components and of gravimetric mixtures are used to create a calibration curve. It is this case that is covered in Section 4 of this Recommended Practice Guide.

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